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101.
Odorant-binding proteins OBP57d and OBP57e affect taste perception and host-plant preference in Drosophila sechellia 总被引:1,自引:0,他引:1 下载免费PDF全文
Despite its morphological similarity to the other species in the Drosophila melanogaster species complex, D. sechellia has evolved distinct physiological and behavioral adaptations to its host plant Morinda citrifolia, commonly known as Tahitian Noni. The odor of the ripe fruit of M. citrifolia originates from hexanoic and octanoic acid. D. sechellia is attracted to these two fatty acids, whereas the other species in the complex are repelled. Here, using interspecies hybrids between D. melanogaster deficiency mutants and D. sechellia, we showed that the Odorant-binding protein 57e (Obp57e) gene is involved in the behavioral difference between the species. D. melanogaster knock-out flies for Obp57e and Obp57d showed altered behavioral responses to hexanoic acid and octanoic acid. Furthermore, the introduction of Obp57d and Obp57e from D. simulans and D. sechellia shifted the oviposition site preference of D. melanogaster Obp57d/eKO flies to that of the original species, confirming the contribution of these genes to D. sechellia's specialization to M. citrifolia. Our finding of the genes involved in host-plant determination may lead to further understanding of mechanisms underlying taste perception, evolution of plant–herbivore interactions, and speciation. 相似文献
102.
Hasegawa S Uematsu K Natsuma Y Suda M Hiraga K Jojima T Inui M Yukawa H 《Applied and environmental microbiology》2012,78(3):865-875
Production of L-valine under oxygen deprivation conditions by Corynebacterium glutamicum lacking the lactate dehydrogenase gene ldhA and overexpressing the L-valine biosynthesis genes ilvBNCDE was repressed. This was attributed to imbalanced cofactor production and consumption in the overall L-valine synthesis pathway: two moles of NADH was generated and two moles of NADPH was consumed per mole of L-valine produced from one mole of glucose. In order to solve this cofactor imbalance, the coenzyme requirement for L-valine synthesis was converted from NADPH to NADH via modification of acetohydroxy acid isomeroreductase encoded by ilvC and introduction of Lysinibacillus sphaericus leucine dehydrogenase in place of endogenous transaminase B, encoded by ilvE. The intracellular NADH/NAD(+) ratio significantly decreased, and glucose consumption and L-valine production drastically improved. Moreover, L-valine yield increased and succinate formation decreased concomitantly with the decreased intracellular redox state. These observations suggest that the intracellular NADH/NAD(+) ratio, i.e., reoxidation of NADH, is the primary rate-limiting factor for L-valine production under oxygen deprivation conditions. The L-valine productivity and yield were even better and by-products derived from pyruvate further decreased as a result of a feedback resistance-inducing mutation in the acetohydroxy acid synthase encoded by ilvBN. The resultant strain produced 1,470 mM L-valine after 24 h with a yield of 0.63 mol mol of glucose(-1), and the L-valine productivity reached 1,940 mM after 48 h. 相似文献
103.
Kondo S Sugaya S Sugawa S Ninomiya M Kittikorn M Okawa K Ohara H Ueno K Todoroki Y Mizutani M Hirai N 《Journal of plant physiology》2012,169(3):234-241
The effects of an abscisic acid (ABA) 8′-hydroxylase inhibitor (Abz-F1) on ABA catabolism, stomatal aperture, and water potential were examined in apple seedlings under dehydration and rehydration conditions. In this study, 9-cis-epoxycarotenoid dioxigenase (MdNCED) and ABA 8′-hydroxylase (MdCYP707A) genes were isolated and their expressions were investigated under dehydration and rehydration conditions. The stomatal aperture decreased up to 4 h after spraying with Abz-F1 and the stomatal aperture in the Abz-F1-treated leaves was generally lower than that in the untreated control-leaves during the dehydration condition. Although the water potential in untreated control-leaves decreased with the progress of dehydration, it was maintained at a higher level in the Abz-F1 treated-leaves than in the untreated control-leaves during dehydration. Endogenous ABA concentrations increased with dehydration in both the Abz-F1 treated- and untreated-control-leaves, but the ABA levels in the Abz-F1 treated-leaves were higher than those in the untreated control-leaves throughout dehydration. In contrast, the phaseic acid (PA) concentrations in the Abz-F1 treated-leaves were lower than those in the untreated control-leaves during dehydration. The expressions of MdNCEDs in the Abz-F1 treated-leaves were lower than those in the untreated control-leaves regardless of the higher endogenous ABA concentrations. Moreover, the expressions of MdCYP707As in the Abz-F1 treated-leaves were also lower than those in the untreated control-leaves. Higher 50% effective concentrations (EC50) and ascorbic acid concentrations were observed in the Abz-F1 treated-leaves, which show that the oxidative damage under dehydration may be reduced by Abz-F1 application.These results suggest that prompt stomata closure is required for survival under dehydration, and Abz-F1 application may therefore be of practical use. The increase of endogenous ABA, which induced prompt stomata closure in Abz-F1 treated-leaves may depend on inhibition of the expression of MdCYP707As. Furthermore, the results showed the close relationship between MdNCEDs and MdCYP707As on ABA catabolism. 相似文献
104.
Kamijo A Sugaya T Hikawa A Yamanouchi M Hirata Y Ishimitsu T Numabe A Takagi M Hayakawa H Tabei F Sugimoto T Mise N Omata M Kimura K 《Molecular and cellular biochemistry》2006,284(1-2):175-182
Background: We reported that urinary L-FABP reflected the progression of chronic kidney disease (CKD). This study is aimed to evaluate the clinical significance
of urinary liver type fatty acid binding protein (L-FABP) as a biomarker for monitoring CKD. Methods: Urinary L-FABP was measured using human L-FABP ELISA kit (CMIC.Co., Ltd., Tokyo, Japan). The relations between urinary L-FABP and clinical parameters were evaluated in non-diabetic CKD (n = 48) for a year. In order to evaluate the influence of serum L-FABP derived from liver upon urinary L-FABP, both serum and urinary L-FABP were simultaneously measured in patients with CKD (n = 73). Results: For monitoring CKD, the cut-off value in urinary L-FABP was determined as 17.4 μg/g.cr. by using a receiver operating characteristics (ROC) curve. Renal function deteriorated
significantly more in patients with ‘high’ urinary L-FABP (n = 36) than in those with ‘low’ L-FABP (n = 12). The decrease in creatinine clearance was accompanied by an increase in urinary L-FABP, but not in urinary protein. Serum L-FABP in patients with CKD was not correlated with urinary L-FABP. Conclusion: Urinary excretion of L-FABP increases with the deterioration of renal function. Serum L-FABP did not influence on urinary L-FABP. Urinary L-FABP may be a useful clinical biomarker for monitoring CKD. 相似文献
105.
Ogawa M Koyanagi J Sugaya A Tsuda T Ohguchi H Nakayama K Yamamoto K Tanaka A 《Bioscience, biotechnology, and biochemistry》2006,70(4):1009-1012
We investigated the cytotoxic activity of 2-substituted naphtho[2,3-b]furan-4,9-diones. We have previously synthesized 33 types of 2-substituted and related compounds, and the cytotoxic activity of these compounds was then examined by a KB cell culture assay. 2-(3-Furanoyl)benzoic acids and 1,4-naphthoquinones had no activity. 2-Acetyl-4,9-dimethoxynaphtho[2,3-b]furan 4 showed low activity. However, parent naphtho[2,3-b]furan-4,9-dione 2 and most 2-substituted derivatives exhibited cytotoxic activity. The parent structure was therefore for cytotoxicity. 2-Formylnaphtho[2,3-b]furan-4,9-dione 11 had particularly potent activity (ED50=0.09 microg/ml). 相似文献
106.
Tanabe H Ito H Sugiyama K Kiriyama S Morita T 《Bioscience, biotechnology, and biochemistry》2006,70(5):1188-1194
The purpose of this study was to examine the effects of dietary indigestible components on mucin secretion in the respective parts of the gastrointestinal tract through their physico-chemical properties. Rats were fed either a control diet or diets containing 5% polystyrene foam (PSF), 5% fructooligosaccharide (FOS), 5% PSF + 5% FOS, or 10% beet fiber for 10 d. Mucins in the small intestine and feces were greater in the PSF, PSF + FOS, and beet fiber groups than in the control and FOS groups. In the cecum, greater mucins were observed in the FOS, PSF + FOS, and beet fiber groups than in the control and PSF groups. None of the dietary treatment was effective on gastric mucins. Cecal mucins were significantly correlated with the cecal pool sizes of total short-chain fatty acids. The correlation between fecal mucins and fecal numbers was also significant. The results suggest that the effect of the bulk-forming property of the dietary indigestible component on mucin secretion is limited to the duct, while fermentability is effective only in the cecum. 相似文献
107.
Sekiya A Fukada S Morita T Kawagishi H Sugiyama K 《Bioscience, biotechnology, and biochemistry》2006,70(8):1987-1991
The effect of dietary eritadenine on the plasma homocysteine concentration was investigated in methionine-induced hyperhomocysteinemic rats. The rats were fed on the control or eritadenine-supplemented (50 mg/kg) diet for 10 d. The animals were then injected with saline or methionine at a level of 100 or 300 mg/kg of body weight, and sacrificed 2 h or a more appropriate time after injection. The methionine injection increased the post-2 h concentration of plasma homocysteine in a dose-dependent manner in the control rats, this increase being significantly suppressed in the eritadenine-fed rats. This effect persisted up to 8 h after the methionine injection. The hepatic concentrations of S-adenosylmethionine and S-adenosylhomocysteine were increased by eritadenine, whereas the hepatic homocysteine concentration was inversely decreased. The cystathionine beta-synthase activity in the liver was increased by eritadenine. It is suggested from these results that eritadenine might suppress the methionine-induced increase in plasma homocysteine concentration by dual mechanisms: slowing the homocysteine production from S-adenosylhomocysteine and increasing the removal of homocysteine due to the enhanced activity of cystathionine beta-synthase. 相似文献
108.
Ito H Yuto S Motoi H Yagishita T Takeya K Sugiyama K Kiriyama S Morita T 《Bioscience, biotechnology, and biochemistry》2006,70(10):2429-2436
We examined in rats whether or not the prolonged ingestion of recrystallized amylose (RCA) would prevent the development of insulin resistance. Rats were fed on a diet containing waxy cornstarch (WCS) as carbohydrate or a diet containing 30% RCA in place of WCS for 18 wk. Glucose tolerance test (GTT) was conducted at every four weeks. On wk 16, the plasma insulin response as assessed by the area under the curve was lower in the RCA diet group than in the WCS diet group. The fasting plasma insulin level tended to increase over time in both groups, but was lower in the RCA diet group on wk 16. An autopsy revealed that the adipose tissue mass and serum free fatty acid concentrations were significantly higher in the WCS diet group. The results suggest that prolonged ingestion of RCA had the effect of slowing the development of insulin resistance through a lower concentration of serum free fatty acids, presumably due to the prevention of adipocyte hypertrophy. 相似文献
109.
Neuroprogenitor cells are an important resource because of their great potential to replace damaged cells in the brain caused
by trauma and disease. Studies have shown that when neuroprogenitor cells are transplanted into the brain they migrate towards
damaged areas, suggesting that these areas express factors that recruit migrating cells. Generally, after neuronal injury,
there is a neuroinflammatory response that results in increased chemokine production. In this present study, we demonstrate
that monocyte chemoattractant protein-1 (MCP-1) significantly induces the migration of NT2 neuroprogenitor cells. Activation
of intracellular cyclic adenosine monophosphate or protein kinase C with forskolin and phorbol 12-myristate 13-acetate, respectively,
was able to completely abolish the MCP-1-induced migration. Contrarily, neither extracellular signal-regulated kinase nor
p38 mitogen-activated protein kinase was required for NT2 cells to respond to MCP-1. Previously, we showed that amyloid precursor
protein (APP) activity increases MCP-1 expression in NT2 cells. We now demonstrate that NT2 cells expressing APP can induce
migration of other neuroprogenitor cells. Utilizing a MCP-1 neutralizing antibody, we discovered that APP-induced migration
was not caused solely by increased MCP-1 production. Interestingly, APP-increased expression of several C–C chemokines: MCP-1,
regulated upon activation, normal T-cell expressed, and secreted (RANTES), and macrophage inflammatory protein alpha (MIP-1
alpha). This demonstrates the unique role APP has in regulating chemokine production, which directly affects cell migration.
Taken together, these data provides greater detail of the chemotactic factors and intracellular signaling that direct neuroprogenitor
cell migration, allowing for better understanding of cell migration during transplantation. 相似文献
110.
Differential adaptation of high- and low-chill dormant peaches in winter through aquaporin gene expression and soluble sugar content 总被引:2,自引:0,他引:2
Suravoot Yooyongwech Sumiko Sugaya Yoshihiko Sekozawa Hiroshi Gemma 《Plant cell reports》2009,28(11):1709-1715
Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter
an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast
and plasma membrane aquaporin genes differed in the bud cushions of the high-chill peach (Prunus persica L. Batsch) cv. Kansuke Hakuto and the low-chill peach cv. Coral. In December and January, when the temperature was lowest
(around 2°C), the increased expression of Pp-γTIP1 and Pp-PIP1 seen in the bud cushions of Kansuke Hakuto may have been related to the concomitant high-soluble sugar content of the cushions
of this cultivar. This relationship may have made the cells highly stable and relatively unaffected by low-temperature stress
owing to the presence of “glasses” that prevented ice nucleation. However, a simpler form of cold protection regulation seemed
to occur in Coral, in which there was no winter increase in Pp-γTIP1 and Pp-PIP1 mRNA and a slow decline in total soluble sugar content in December and January. These results suggested that Pp-γTIP1 and Pp-PIP1, respectively, play important roles in intra- and intercellular membrane transport, enhancing cold resistance in the bud
cushions of high-chill cultivars. In addition, Pp-δTIP1 and Pp-PIP2 mRNA increased at the end of endodormancy in both cultivars. This change may be induced by endodormancy-release signals and
the resumption of bud activity in both cultivars. 相似文献